Coding

Part:BBa_K1021005:Design

Designed by: Swati Sureka   Group: iGEM13_Cornell   (2013-08-30)


Cre recombinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The cre cds, without any flanking elements, was amplified from its original vector and transferred to pSB1C3 for further usage.


Source

pBS185 CMV-Cre plasmid from Addgene

References